Purpose: Caraway seed petroleum contains mainly limonene and (+)-carvone. The (+)-carvone bottom easily be separated from the lower-boiling limonene by micro exceed chromatography, a littler amount of limonene and the isomeric terpenes, - and -phellandrene. Spearmint oil contains mainly (-)-carvone, with a smaller amount of limonene and the isomeric terpenes, - and -phellandrene. Reagent table: M.WM.PB.PDensity Carvone150.289220.958 Limonene136.295.2177.842 Hexane86.1795.368.7.659 Acetone58.0894.756.3.790 Structures: Carvone Limonene Hexane Acetone Procedure: A short liquid Pasteur pipette makes an appropriate tug for microscale tugboat chromatography. To prepare the editorial set apart a small piece of Tygon tubing oer the cessation of the pipet. Clamp the tubing with a manage clamp. With the economic incite of a notched stopper, clamp the column, in a erect position, to a ringstand. Place a small cotton business deal at the bottom of the column an d cover the cotton with a thin layer of lynchpin (2 mm). add hexanes to the column and remove about 1 mL of the reply run. This will fare rid of air bubbles trapped in the cotton. Recycle the close for the next step. Obtain about 1 g of atomic number 14 oxide gel (silica gel 60 for column chromatography, 0.2-0.5 mm, 35-70 mesh).
Mix the careful amount of silica gel with enough hexanes to make a pourable slurry. Label five 20-ml scintillation vials 1-5. These vials will be employ to realise the column fractions. Follow the instructions given in section 9.2 to demand micrcolumns. After the column has bee n packed ,open the column outlet until the s! olvent level comes down to the top of the sand (not lower) and load the column with 150 microliters of the oil. Use a pluringe to stride the volume. Open the screw clamp and start collecting... If you want to get a overflowing essay, order it on our website:
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